Posted on Sunday, September 30, 2012 at 12:32PM by The GenePattern Team
In GenePattern, you use the ComparativeMarkerSelection module to identify the genes (if any) that are differentially expressed between two phenotype classes. Typically, this is a three-step process:
Posted on Saturday, September 15, 2012 at 12:29PM by The GenePattern Team
In cancer genomics, copy number change is one of the hallmarks of the genetic instability common to most human cancers and loss of heterozygosity (LOH) of tumor suppressor genes is a crucial step in the development of sporadic and hereditary cancer (Monti, 2005). Using modules available in GenePattern, you can compute SNP copy number and LOH based on Affymetrix SNP chip data for paired target/normal samples and then view them in the Integrative Genomics Viewer (IGV). The following modules are used for this computation, with IGV at the end for viewing the results:
SNPFileCreator converts the .CEL files from an Affymetrix array into a GenePattern .SNP file. Raw data for the probes in each SNP probe set are converted to a single intensity value per SNP using one of four modeling algorithms: Average Difference, PM/MM Difference Model (dChip, the default), Median Probe, or Trimmed Mean....
Posted on Thursday, August 30, 2012 at 12:23PM by The GenePattern Team
The Integrative Genomics Viewer (IGV) is a high-performance visualization tool for interactive exploration of large, integrated datasets. It supports a wide variety of data types including sequence alignments, microarrays, and genomic annotations. Up until recently, this tool was only available outside of , though it did accept GenePattern file formats. IGV can now be launched from a module available on the GenePattern Server or downloaded from the GenePattern Repository.
With this new development, users can pass their GenePattern result files directly to IGV through GenePattern.
The GenePattern IGV module launches the same application that is available from the IGV website. If you are a user of both the client IGV (either launching from the IGV website or your desktop) and GenePattern, this means you are using the same version of IGV complete with your preferences, home directory, saved genomes, and other such IGV saved presets. For all users,...